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KMID : 0881720160310010021
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Journal of Food Hygiene and Safety
2016 Volume.31 No. 1 p.21 ~ p.27
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Production of Monoclonal Antibodies against Vibrio parahaemolyticus and Development of High Sensitive Immuno-Selective Filtration Method
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Kim Jeong-Sook
Choi Young-Dong
Shim Won-Bo
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Abstract
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The objectives of this study are to produce monoclonal antibodies (MAbs) against Vibrio parahaemolyticus and to develop an immuno-selective filtration (ISF) method for the rapid and sensitive detection of V. parahaemolyticus. The characterization of the MAb produced from HKVP 4H9-9 hybridoma cell was validated by enzyme-linked immunosorbent assay (ELISA) and western blot. The produced MAb was specific to V. parahaemolyticus and showed weak cross-reaction to V. alginolyticus, V. vulnificus and Staphylococcus aureus. After optimization of the method, 5 × 101 cell/mL of V. parahaemolyticus in a pure culture could be detectable. Although weak crossreactivity to V. vulnificus, V. alginolyticus and Staphylococcus aureus was observed, the ISF was confirmed to be highly specific to V. parahaemolyticus. Especially, the ISF showed the most sensitivity compared to the immunoassays currently reported is easier to perform and quicker than ID-ELISA.
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KEYWORD
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Vibrio parahaemolyticus, monoclonal antibody, ELISA, immuno-selective filtration
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